The purpose of identifying the sequence of the deoxyribonucleic acid chain is to identify the nature of a particular set of the gene which can be utilized in medical sciences for its further development. Such a process is carried out in multiple ways, including the chain termination method that involves the termination of the deoxyribonucleic acid. A single-stranded deoxyribonucleic acid chain synthesized in vitro is first polymerized into double strands with primersâ€™ help. After forming a double-stranded structure, the sequence is identified with the help of a template strand.
The other method is chemical degradation in which chemical acts on the double-stranded structure of deoxyribonucleic acid on specific positions helping in the identification of the location of nitrogenous bases.
Another method called automated sequencing is also performed for genomic sequencing, which is carried out by radioactive labeling. The primers having the fluorescent ability are added, and the location of specific nucleotide is identified. The sequencing method, which is carried on a vast scale, is known as genome sequencing that utilizes the process of fragmentation of the genomic material and then sequenced with the help of a vector.
The Genomic Sequencing Procedure
The sequencing processes apart from the genomic sequencing are carried on the entire length of the genome, which is practically impossible; hence, the entire genomic sequence is digested and fragmented into short strands that are maintained by specific vectors. The genome sequencing using this method can be carried out by the shotgun approach and the clone contig approach. The shotgun approach includes the cloning of the fragment vectors, and the clones are then overlapped. The sequence of the overlaps on the cloned vector is further studied to identify the sequence. Overall, the clone contig approach relies on the formation of contigs, which are continuous sequences of deoxyribonucleic acids in which the contigs are eventually formed by the successive overlapping of alternative clones over each other in which the same overlapping sequences can be identified. A non-specialist of the arrangement of nitrogenous bases in the genome could be formed.
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