Triglyceride is a naturally occurring hydrophobic lipid composed of a glycerol backbone and fatty acids. Esterification of glycerol and a single long-chain fatty acid produce monoglyceride, whilst reaction with two such fatty acids produce diglyceride, and reaction with three fatty acids results in the formation of triglyceride. Triglycerides are a significant component of oils and fats of both plant and animal origin.
Esterification of glycerol with saturated fatty acids results in the synthesis of crystallized triglycerides with a high melting point (such as fats). In contrast, esterification with unsaturated fatty acids produces triglycerides with a low melting point (such as oils). The three fatty acids in triglyceride reaction may or may necessarily be the same. The type of fatty acids used, chain length, polarity, and type of bonds influence the physicochemical properties of triglycerides. The synthesis of triglycerides primarily involves the formation of triple ester bonds. The three hydroxyl groups present in glycerol react with three carboxyl groups of three fatty acids. The hydroxyl groups are replaced, and the oxygen atoms connect the glycerol carbons with the three fatty acids to produce three ester bonds. Along with the formation of three ester bonds, three hydroxyl groups that are released from glycerol combine with three hydrogen ions released from three fatty acid chains to produce three water molecules.
Triglyceride metabolism and hydrolysis
Enzymes mediate the metabolism of triglycerides. Triglycerides react with water molecules and undergo the hydrolysis process. The hydrolysis of triglycerides in the presence of pancreatic enzyme lipases under acidic pH leads to the breakdown of triglycerides into fatty acids and glycerol. Hydrolysis of triglycerides is the opposite reaction of triglyceride synthesis. It involves the consumption of water molecules to dissolve the ester bonds in the triglyceride and split it into glycerol and fatty acids. This phenomenon is also known as lipolysis.
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