When auxins are supplemented in the medium relatively lower than the concentrations of cytokinins they induce root formation. For example, IBA (indole-3-butyric acid) at micromolar levels (9.8 micromolar) generally induce root formation.
Most of the auxins like that of IAA, IBA, and NAA at 0.1-1mg/lit concentrations induce multiple root formation. For example, culturing of Luquidamber styraciflua requires 200mg/li of IBA than the cytokinin concentration which favors the root formation.
In some cases, the vitamin concentrations of certain vitamins (for example vitamin-D) affect the functionality of IBA in the tissue culture medium. It has an effect on the root formation in the adventitious roots of cultures of Populus tremula.
Role of cytokinin in plant growth and development:
The physiological effects of cytokinin promote embryogenesis in plant tissue cultures. The inclusion of the liquid content of coconut promotes the plant cell cultures that exclusively contain kinetin.
Most of the cytokinins induce cell division during growth and 200 types of naturally occurring cytokinins have been identified. This class of hormone is found in higher amounts in most parts of the cultured plants.
These plant parts include the embryos, roots, leaves, and fruits. A delay in the leaf senescence and the mitotic cell division is found to be greatly influenced by cytokinin. Cytokinin induces root differentiation and shootsâ€™ meristematic differentiation.
The principle of apical dominance is established in tissue cultured plants and is established by the balance auxins that prevent the formation of lateral buds and the cytokinin ratio on the other hand promotes lateral bud formation.
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